Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | acetyl-CoA is required for activation | Escherichia coli | |
PanZ | analysis of the protein complex between Escherichia coli PanD and PanZ in vitro using isothermal titration calorimetry and mass spectrometry and thereby determine the stoichiometry and affinity of the components of this protein complex, high affinity 4:4 heterooctameric complex, overview. Non-covalent interaction between the acetyl group of acetyl-CoA and the PanD zymogen may be required to induce activation | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene panD, expression of His-tagged enzyme mutant T57V and PanZ in Escherichia coli strain DELTApanD DELTApanZ (DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
T57V | site-directed mutagenesis | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | Escherichia coli | - |
beta-alanine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene panD | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme mutant T57V and PanZ from Escherichia coli strain DELTApanD DELTApanZ (DE3) by nickel affinity chromatography and gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Escherichia coli | beta-alanine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Aspartate alpha-decarboxylase | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
additional information | regulatory mechanisms for PanD activation and inactivation in vivo, overview | Escherichia coli |